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Hepatitis C Research

2002 & 2003 Articles

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  Induction Dose of PEG-Intron Is More Effective Than Fixed Dose in Combination with Ribavirin for Chronic HCV Patients Nonresponsive to a Previous Treatment
  Retreatment with PEG-Intron Plus Ribavirin Shows Limited Benefit in Patients with Chronic Hepatitis C Not Responding to Previous Treatment with Standard Interferon and Ribavirin
  Interim Results of Observational Study of Patients Treated for Chronic Hepatitis C in France
  Early Virologic Response (EVR) at Week 8 Is More Predictive of Sustained Response to Pegylated Interferon/Ribavirin Therapy Than at Week 12
  Liver Fibrosis Regresses Better with PEG-Intron and Ursodeoxycholic Acid Treatment Than with Spontaneous Recovery
  Pharmacokinetics of Pegasys Compared to PEG-Intron in Treatment-Naïve Patients with Chronic Hepatitis C

 

 
Induction Dose of PEG-Intron Is More Effective Than Fixed Dose in Combination with Ribavirin for Chronic HCV Patients Nonresponsive to a Previous Treatment

Abstract Summary


The efficacy of PEG-Intron (pegylated interferon alfa-2b) plus ribavirin in patients resistant to interferon monotherapy or standard combination therapy is unknown.

Conducted in France at multiple medical centers, the present study compared the efficacy and safety of two dose regimens of PEG-Intron (PEG-IFN) plus ribavirin (RBV) in non-responders to interferon monotherapy or to standard combination therapy.

233 patients with chronic hepatitis C have been randomized to receive: group 1: PEG-IFN 2.0 mcg/kg/wk , RBV 800 mg daily x 8 weeks, then PEG-IFN 1.0 mcg/kg/wk plus RBV 800 mg daily x 40 weeks, OR group 2: PEG-IFN 1.0 mc/kg/wk plus RBV 800 mg daily x 48 weeks.

Baseline characteristics included: genotype 1: 69%, HCV RNA titer > 800000 IU/ml: 66%, cirrhosis: 24%, prior monotherapy: 42%. 226 patients have completed the first 48 weeks of treatment. The virological response rate was higher in group 1: 32% (36/114) than in group 2: 18% (20/112).

The rate of virological response was higher among non-responders to prior monotherapy in group 1: 44% (21/48) than in group 2: 17% (8/47), and among patients with genotype non-1: 58% (21/36) in group 1 vs 24% (8/34) in group 2.

Conclusion: At week 48 of therapy, the induction dose is more effective than the fixed dose in non-responders. The benefit of the induction regimen is more apparent in patients with prior IFN monotherapy and in those with genotype non-1 infection.                                                                                
03/28/03

Reference
M Chousterman and others. EFFICACY OF PEGYLATED INTERFERON ALPHA-2B IN COMBINATION WITH RIBAVIRIN IN PATIENTS WITH CHRONIC HEPATITIS C NON-RESPONDERS TO A PREVIOUS TREATMENT. Abstract 4408.00. Abstracts of the 38th Annual Meeting of the European Association of the Study of the Liver (EASL).

 

 

  Retreatment with PEG-Intron Plus Ribavirin Shows Limited Benefit in Patients with Chronic Hepatitis C Not Responding to Previous Treatment with Standard Interferon and Ribavirin

Abstract Summary


In interferon (IFN) non-responders with chronic hepatitis C, retreatment with standard interferons combined with ribavirin showed sustained virologic response rates of 20-30%. Only limited data with conflicting results are available concerning the efficacy of antiviral retreatment in patients not responding to a previous treatment with standard interferons combined with ribavirin

The aims of the present German trial were to evaluate efficacy and safety of a retreatment with PEG-Intron (peginterferon alfa-2b) plus ribavirin in 240 patients (162 males, 78 females, mean age 45.5 years) not responding to previous antiviral treatment with standard interferons combined with ribavirin.

Patients received PEG-Intron 100 mcg/week subcutaneously (sc) for 8 weeks followed by 50 mcg/week sc for 40 weeks in combination with ribavirin 800 mg/d for 48 weeks. Treatment was discontinued in patients with detectable serum HCV RNA after treatment week 24.

A virologic end-of-treatment response was achieved in 25/240 (10.4%) patients while after a follow-up period of 24 weeks a sustained virologic response was observed in 15/240 (6.3%) patients.

Patients infected with HCV genotype non-1 were more likely to respond to antiviral retreatment than patients infected with HCV genotype 1 (6.8% vs. 17%).

Conclusions: antiviral retreatment with PEG-Intron plus ribavirin in this flat and low dose showed only a limited therapeutic efficacy in patients with chronic hepatitis C not responding to previous treatment with standard interferons and ribavirin.

In these patients, virologic response rates may be improved by administering PEG-Intron and ribavirin bodyweight adapted or by the addition of other antiviral agents.                   

03/28/03

Reference
G Teuber and others. CONTROLLED TRIAL WITH PEGYLATED INTERFERON-ALPHA2B PLUS RIBAVIRIN RETREATMENT IN PATIENTS WITH CHRONIC HEPATITIS C NOT RESPONDING TO A PREVIOUS ANTIVIRAL TREATMENT WITH STANDARD INTERFERONS COMBINED WITH RIBAVIRIN. Abstract 3904.00. Abstracts of the 38th Annual Meeting of the European Association of the Study of the Liver (EASL).

Interim Results of Observational Study of Patients Treated for Chronic Hepatitis C in France

Abstract Summary


The objective of this observational study undertaken in France is to evaluate medical practices for the treatment of chronic hepatitis C, and to assess the observance, tolerance and efficacy of treatment outside clinical trials.

A sample of 50 specialists working in various settings participated in the study. All consecutive patients who were prescribed authorized antiviral drugs were enrolled. Demographic, epidemiological and virological data were collected before treatment; follow up was made according to the habits of each prescriber and included information on acceptability of the treatment, dose modifications and their motive.

Between April 1st and December 31st 2001, 578 patients were enrolled. The epidemiological, virological and histological features of this group were similar to those patients usually treated in France. Fifty-seven percent of patients were treatment-naive, 16% were relapsers and 27% were non-responders.

In 93% of cases, treatment consisted of PEG-Intron (peginterferon alfa-2b/PEG-IFN) and ribavirin at recommended doses: on average 1.4 mcg/kg of PEG-IFN and 13 mg/kg of ribavirin (16 to 11 mg/kg according to weight), irrespective of gender, genotype and the existence of a previous treatment. All patients have been followed at least 24 weeks.

Currently, 26 patients (4.5%) have withdrawn from treatment; 110 (19%) patients have needed a dose reduction of PEG-IFN and/or ribavirin. The acceptability of treatment was good for 36% of patients, moderate or poor for 64% of patients. The latter were more often women (44 vs 34%), they were older (48.8 vs 44.6 yrs), for an equivalent dose of PEG-IFN and ribavirin. Nevertheless, a dose reduction was prescribed in only 32% of patients with moderate or poor tolerance, these patients receiving an average higher initial dose of PEG-IFN (1.5 vs 1.4 mcg/kg, p=0.01).

Virological response on treatment is now available for 299 patients: HCV RNA is undetectable in 199 of them (66.6%, IC95% 61.2-71.9).

Conclusion: The interim results of this observational study show that

  • Most patients are treated according to recommendations;
  • Early withdrawal of combination therapy remains rare; and
  • Initial regimen is often continued despite moderate or poor tolerance, which may be important to attain virological response.

03/28/03

Reference
F Roudot-Thoraval and others. OBSERVATIONAL STUDY OF PATIENTS TREATED FOR CHRONIC HEPATITIS C IN FRANCE. Abstract 3867.00.
Abstracts of the 38th Annual Meeting of the European Association of the Study of the Liver (EASL).

Early Virologic Response (EVR) at Week 8 Is More Predictive of Sustained Response to Pegylated Interferon/Ribavirin Therapy Than at Week 12

Abstract Summary

The aim of this study was to assess the predictive value of early virologic response (EVR) in patients receiving combination therapy with PEG-Intron (peginterferon alfa-2b/PEG-IFN) plus ribavirin (RIBA).

The study enrolled 81 patients: 43 naive and 38 previously treated (PT) with the combination of Peg-IFN  + RIBA were studied. Serum HCV RNA was measured at baseline, weeks 1, 2, 4, 8, and 12, end of treatment and 24 weeks after treatment by VERSANT HCV 3.0 (bDNA) assay and VERSANT HCV RNA Qualitative Assay (HCV TMA). EVR was defined as ≥ 2 log drop of HCV RNA with bDNA or undetectable HCV RNA with TMA during the first 12 weeks of therapy.

The kinetic slope of HCV RNA in SR was faster in naive than in PT patients.

The positive Predictive Value (PPV) of SR according to EVR was 96%, 86%, 80% and 80% in naive and 71%, 67%, 60% and 63% in PT at week 2, 4, 8 and 12, respectively.

The negative Predictive Value (NPV) of SR according to EVR was 25%, 9%, 0% and 0% in naive and 19%, 20%, 18% and 17% in PT, at week 2, 4, 8 and 12, respectively. PPV of SR according to TMA was 100%, 94%, 85% and 88% in naive and 0%, 94%, 100% and 86% in PT at week 2, 4, 8 and 12, respectively.

NPV of SR according to TMA was 66%, 52%, 42% and 17% in naive and 48%, 41%, 28% and 23% in PT, at week 2, 4, 8 and 12, respectively. The more accurate predictive values for SR were TMA negative at week 4 or EVR at week 8.

Conclusions: Prediction of SR assessed by ≥ 2 log drop (bDNA) or undetectable (TMA) HCV RNA might be done as early as week 2 in naïve patients and as early as week 4 in PT patients.

Prediction of no SR is optimal at week 8 in naive and somewhat less accurate in PT patients. Therefore treatment status should be considered when assessing patients response to therapy.

03/28/03

Reference
M Martinot-Peignoux and others. EARLY VIROLOGIC RESPONSE (EVR) AT WEEK 8 IS MORE PREDICTIVE OF SUSTAINED RESPONSE (SR) TO PEGYLATED COMBINATION THERAPY THAN AT WEEK 12. Abstract 4232.00. Abstracts of the 38th Annual Meeting of the European Association of the Study of the Liver (EASL).

Liver Fibrosis Regresses Better with PEG-Intron and Ursodeoxycholic Acid Treatment Than with Spontaneous Recovery

Abstract Summary


Fibrosis and cirrhosis are common complications of chronic liver diseases. An imbalance between fibrogenesis and fibrolysis results in scarring of the liver parenchyma. In the current study, researchers in Turkey investigated the possible antifibrotic effectiveness of a newly modified interferon molecule peginterferon a2b (PEG-IFN) which has better antiviral activity and ursodeoxycholic acid (UDCA).

Liver fibrosis was established on 60 male Sprague Dawley rats with CCl4 in 12 weeks. After cessation of CCL4 Group I was left for spontaneous recovery. Group II was treated with Peg-Intron (PEG-IFN 1.5 mcg/kg/week), Group III with UDCA 25 mg/kg/day and Group IV with combination of both drugs. All rats were killed at week 16.

Histopathologic fibrosis scores, tissue hidroxyprolin, TIMP-1 and MMP-13 levels were determined. Apoptosis was detected by TUNEL staining. Fibrosis scores were lower in both Group II, III and IV than Group I. Tissue hidroxyprolin levels were significantly decreased in Group II, III and IV when compared to Group I, respectively). Lower liver TIMP-1 and higher MMP-13 levels were measured in Group II, III, and Group IV than Group I (p=0.007, p=0.05, p<0.001 for TIMP and p<0.001, p=0.02, p<0.001, for MMP, respectively). Apoptosis was significantly increased in Group II, III and IV when compared to Group I (p<0.001, p<0.05, p<0.05, respectively). There was significantly higher apoptosis in Group II than III and IV (p<0.06, p<0.05, respectively).

Conclusion: Treatment with both peginterferon alfa-2b and ursodeoxycholic acid improves CCL4 induced rat liver fibrosis. Significantly higher effects can be obtained using these agents in combination.                  

03/28/03

Reference
I Tasci and others. LIVER FIBROSIS REGRESSES BETTER WITH PEGINTERFERON ALPHA AND URSODEOXYCHOLIC ACID TREATMENT THAN SPONTANEOUS RECOVERY. Abstract 3321.00.
Abstracts of the 38th Annual Meeting of the European Association of the Study of the Liver (EASL).

 

   
Pharmacokinetics of Pegasys Compared to PEG-Intron in Treatment-Naïve Patients with Chronic Hepatitis C
 
Abstract Summary

The currently available pegylated interferon (PEG-IFN) formulations, PEG-IFN alfa-2a (40KD, PEGASYS) and PEG-IFN alfa-2b (12KD, PEG-Intron), have different pharmacokinetic profiles. The aim of this current study, conducted at the University of Pavia, Italy, was to compare the pharmacokinetics of the two PEG-IFNs in patients with chronic hepatitis C (CHC).

30 treatment naive patients with CHC and persistently elevated ALT levels have been randomized to receive 180 mcg PEGASYS once-weekly (n=16) or 1.0 mcg/kg once-weekly of PEG-Intron (n=14). Serum concentrations of both PEG-IFNs were measured at baseline and 24, 48, 120 and 168 hours after administration using a quantitative sandwich ELISA (lower limit of detection 125 pg/ml).

Serum concentration of PEG-Intron achieved maximum levels at 24 hours after injection and decreased rapidly until 120 hours. Drug was undetectable 120 and 168 hours after injection in 7 (50%) and 11 (78%) subjects, respectively. In contrast, PEGASYS concentrations increased continuously overtime, reaching maximum levels from 48 to 168 hours.

Conclusions: The investigators conclude, “Our data demonstrate substantial differences in plasma concentration profiles between PEGASYS and PEG-Intron. Five days after injection concentrations of PEG-Intron are marginal or undetectable, while those of PEGASYS remain stable overtime.

“These findings suggest that PEG-Intron administration should be intensified to twice weekly to avoid "blips" in viral replication. Differences in pharmacokinetics could explain the differences observed in HCV decay.”                                                                  

03/24/03

Reference
R Bruno and others. PHARMACOKINETICS OF PEGINTERFERON ALFA-2A (40KD, PEGASYS) COMPARED TO PEGINTERFERON ALFA-2B (12KD, PEGINTRON) IN NAIVE PATIENTS WITH CHRONIC HEPATITIS C (CHC). Abstract 4203.00. Abstracts of the 38th Annual Meeting of the European Association of the Study of the Liver (EASL).